منابع مشابه
Transgenic livestock: premises and promises.
Microinjection of DNA constructs into pronuclei of zygotes has been the method of choice for the generation of transgenic livestock. However, this procedure is characterized by low efficiency (1-4% transgenic offspring), random integration and variable expression of the transgene as well as a considerable proportion of mosaicism. Furthermore, it is extremely time consuming and costly. As a cons...
متن کاملResource requirements for transgenic livestock research.
Transgenic research usually involves a large investment in carrying out a series of low-efficiency steps to produce a few transgenic founder animals, and then characterizing a transgenic line of animals derived from each founder. There is considerable variation in phenotype among transgenic lines made with the same DNA construct. In the case of livestock species, to produce one founder animal t...
متن کاملTransgenic livestock for agriculture and biomedical applications
The classical definition of a genetically engineered or “transgenic” animal would be “those animals modified by recombinant DNA (rDNA) techniques” according to the U.S. Food and Drug Administration (United States Food and Drug Administration, Guidance #187, 2009). Thus, the introduction or deletion of any gene by using recombinant DNA would fall into this category. Since the first reports of st...
متن کاملGeneration of transgenic livestock by somatic cell nuclear transfer
The ability to produce transgenic animals through the introduction of exogenous DNA has existed for many years. However, past methods available to generate transgenic animals, such as pronuclear microinjection or the use of embryonic stem cells, have either been inefficient or not available in all animals. More recently, somatic cell nuclear transfer has provided a method to create transgenic a...
متن کاملThe changing role of cell culture in the generation of transgenic livestock.
Transgenesis may allow the generation of farm animals with altered phenotype, animal models for research and animal bioreactors. Although such animals have been produced, the time and expense involved in generating transgenic livestock and then evaluating the transgene expression pattern is very restrictive. If questions about the ability and efficiency of expression could be asked solely in vi...
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ژورنال
عنوان ژورنال: Bioscientifica Proceedings
سال: 2019
ISSN: 2515-0227
DOI: 10.1530/biosciprocs.9.018